Yüzüncü Yıl Üniversitesi Tıp Fakültesi Kalp Damar Cerrahisi Anabilim Dalı

Aim: Lung injury may occur after the surgery involving the temporary aortic cross clamping with subsequent ischemia/reperfusion of the lower extremities. Activation of the neutrophils is known to play an important role in the ischemia/reperfusion injury .The aim of this study was to determine whether ischemia/reperfusion could have an important role on lung injury and to determine whether heparin could have a preventive effect on this injury in the rat model of abdominal aorta cross clamping. Methods: Thirty Sprague-Dawley type rats were randomized into three groups. Rats in the group A (n:10) were undertaken laparotomy and closed after dissection of the abdominal aorta without cross clamping. Rats in the group B (n:10), were also undertaken laparotomy and abdominal aorta was dissected and clamped for 3 hours, then removed for reperfusion and abdomen was closed. In the group C rats (n:10), 500 u/kg deltaparin sodium was injected subcutaneously one hour before operation and subjected to the same surgical procedures as for group B rats. All rats were sacrificed and their lungs were removed for MDA analysis and histological examination. Results: Rats in the group B have significantly higher neutrophil sequestration and lipid peroxidation product (MDA) in the lungs. The rats of group C exhibited lesser neutrophil sequestration and MDA levesl than group B. But the differencse were not statistically significant. Conclusion: It is concluded that cross clamping of the abdominal aorta increases the neutrophil sequestration and augments the lung injury process. Heparin exhibited not significantly lesser neutrophil sequestration and MDA level.